In biology and medicine, besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample. Serial dilutions are widely used in experimental sciences, including biochemistry, pharmacology, microbiology, and physics. A tenfold dilution for each step is called a logarithmic dilution or log-dilution, a 3.16-fold (10 0.5-fold) dilution is called a half-logarithmic dilution or half-log dilution, and a 1.78-fold (10 0.25-fold) dilution is called a quarter-logarithmic dilution or quarter-log dilution. Serial dilutions are used to accurately create highly diluted solutions as well as solutions for experiments resulting in concentration curves with a logarithmic scale. A ten-fold serial dilution could be 1 M, 0.1 M, 0.01 M, 0.001 M. Usually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion. How Do You Make A 2 Fold Serial DilutionĪ serial dilution is the stepwise dilution of a substance in solution.Prepare A Serial 2 Fold Dilution To 1024.
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